Part:BBa_K3726072
Lv.1 AcrbV2 - C
This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726065” L0_AcrbV2 which codifies a membrane transporter subunit , which has been optimized for the specific secretion of n-butanol. Within our project we have used AcrbV2 to enhance n-butanol secretion through cell membrane in order to cut down on toxicity generated by itself.
The part is flanked by two connectors that will allow the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 Bba_K2560066, and in its downstream region 3'Con2 Bba_K2560071 . Expression is controlled by the medium-strong synthetic constitutive promoter J23119 “BBa_K2560031” and by the strong synthetic RBS, BCD2(mod) “BBa_K3726086”
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 896
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 436
Illegal NheI site found at 459
Illegal NheI site found at 625
Illegal PstI site found at 896 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 896
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 896
Illegal NgoMIV site found at 2032
Illegal NgoMIV site found at 3548
Illegal AgeI site found at 109 - 1000COMPATIBLE WITH RFC[1000]
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